The overarching goal of Dhingra lab is to understand the molecular mechanisms associated with pathogenesis and azole drug tolerance in invasive pulmonary aspergillosis. We use molecular, biochemical and cellular approaches as well as murine models to understand the role of ncRNAs in pathobiology and azole drug tolerance of Aspergillus fumigatus.
Theme 1: Molecular Mechanisms Governing Azole Drug Tolerance
To understand and improve azole drug treatment in clinic, we are defining the mechanisms allowing resilient fungal growth in steady drug concentration without a change in its Minimum Inhibitory Concentration (MIC), phenomenon termed as drug tolerance. To this end, we seek to identify regulators and pathways governing azole tolerance with a focus on ncRNAs.
Theme 2: Role of ncRNAs in Invasive Pulmonary Aspergillosis
Our lab is focused on understanding the role of ncRNAs in pathobiology of Aspergillus fumigatus. We use molecular and biochemical approaches to understand the structure-function relationship of ncRNAs and their role in pathobiology of A. fumigatus.
Theme 3: Defining ncRNA interaction network and its function
We use cutting edge RNA centric techniques to define and characterize the interacting partners of ncRNAs including DNA, RNA and proteins.